![]() ![]() ![]() Failing in that may spoil any attempt of doing deconvolution, because deconvolution works on the PSF scale. ![]() As the Point Spread Function (PSF) is the basic "brick" of which the images are "built", one should record details at least on the scale of the PSF to gather all the available information. Ideal means necessary to capture all the information. Images obtained with sampling distances larger than those established by this rate are not well sampled. It is recommended to sample the image at a rate close to the ideal Nyquist Rate. The ideal Sampling Density during image acquisition depends on the optics of the microscope. ![]()
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